The complete chloroplast genome sequence of Spiraea japonica var. acuminata Franch. (Rosaceae)

Abstract Spirea japonica var. acuminata Franch. (Rosaceae) is a Chinese herbal medicine distributed in southwest and east China. The first complete chloroplast genome of Spirea japonica var. acuminata Franch. was assembled and reported in this study. The genome is 153,822 bp in length and contained 125 encoded genes in total, including 80 protein-coding genes, eight ribosomal RNA genes, and 37 transfer RNA genes. The phylogenomic analysis showed that Spirea japonica var. acuminata Franch. was closely related to Spirea blumei, Spirea trilobata, Spirea mongolica and Spirea insularis according to the current sampling extent.

Spirea japonica L. f. (1782) is a perennial shrubby species with pink flowers occurring in clusters at the tips of branches. It is widely dissimilar in 14 intraspecific varieties. Spirea japonica var. acuminata Franch. is one variant of Spirea japonica L. f. which is a Chinese herbal medicine classified in the Rosaceae. It is mainly distributed in southwest and east China (He et al. 2001;Zhang et al. 2006). The extracts from these plants were found to be bioactive for treating cough, anti-inflammation, headache, and analgesia (Li et al. 2002). In this study, we assembled and reported the first complete chloroplast genome of Spirea japonica var. acuminata Franch. The cumulative data will provide potential genetic resources for evolution of Rosaceae.
The leaves of Spirea japonica var. acuminata Franch. were collected from Hangzhou, Zhejiang, China (GPS: E120 14 0 14.95 00 , N30 23 0 58.95 00 ). The specimen and extracted DNA was deposited at the College of Life Sciences and Medicine, Zhejiang Sci-Tech University (Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, http://sky.zstu.edu.cn/, Qiu-Ling He and qlhe@zstu.edu.cn) under the voucher number ZSTULSM0001. The total genomic DNA was extracted from its fresh leaves using the CTAB method in accordance with the manufacturer's instructions. The plastome sequences were generated using the Illumina HiSeq 2500 platform (Illumina Inc., San Diego, CA). In total, ca. 20.6 million high-quality clean reads (150 bp PE read length) were generated with adaptors trimmed. These clean data were de novo assembled to complete chloroplast genome using GetOrganelle (Jin et al. 2020).The assembled cp genome was annotated using Geneious v11.1.5 (Drummond 2012) with Spirea trilobata plastome (GenBank: MW822176) as a reference.

Author contributions statement
Qi Wang and Min-min Chen: conception and design, analysis and interpretation of the data, the drafting of the paper. Xiafang Hu: Data analysis. Rui-hong Wang: supervision. Qiu-ling He: review and editing, funding acquisition. All authors have agreed to be accountable for all aspects of the work..

Disclosure statement
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper..

Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI (https://www.ncbi.nlm.nih.gov) under the Accession no. MZ981784. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA759429, SRR15685682, and SAMN21168751, respectively.